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KMID : 1123920140280060593
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Korean Journal of Oriental Physiology and Pathology
2014 Volume.28 No. 6 p.593 ~ p.600
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Anti-inflammatory Effect of Euphorbiae kansui Radix Extract in Lipopolysaccharide-stimulated Mouse Peritoneal Macrophages
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Kim Jeung-Beum
Kyung Hyuk-Su
Kang Hee
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Abstract
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This study is aimed to investigate the anti-inflammatory effect of Euphorbiae kansui radix methanol extract (ERE) in lipopolysaccharide(LPS)-stimulated mouse peritoneal macrophages. Peritoneal macrophages were obtained from thioglycollate-injected Balb/c mice. Cells were stimulated with LPS or LPS plus interferon-gamma (IFN-γ) in the presence of ERE and various inflammatory markers were assayed. Finally, LPS-induced signaling molecules were measured. ERE up to 400 ㎍/㎖, was not cytotoxic to ERE inhibited LPS/IFN-γ-induced nitric oxide (NO), inducible NO synthase. ERE also reduced the levels of cyclooxygenase-2 and the proinflammatory cytokines such as tumor necrosis factor-α, interleukin(IL)-6 and IL-12. The inhibitory effect of ERE on LPS-induced IκBα degradation was weak but phosphorylation of JNK, p38 and ERK1/2 was strongly suppressed. Our data indicated that the anti-inflammatory effect of ERE in LPS-stimulated macrophages was partly mediated by its inhibition of JNK, p38 and ERK1/2.
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KEYWORD
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Euphorbiae kansui radix, anti-inflamatory effect, macrophages, lipopolysaccharide
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